1609-47-8 | Diethyl pyrocarbonate

Packsize	Purity	Availability	Price	Discounted Price
5g	98%	in stock	$25.00	$18.00
25g	98%	in stock	$42.00	$30.00
100g	98%	in stock	$99.00	$69.00
500g	98%	in stock	$387.00	$271.00

Description

• Catalog Number: AA87253
• Chemical Name: Diethyl pyrocarbonate
• CAS Number: 1609-47-8
• Molecular Formula: C6H10O5
• Molecular Weight: 162.1406
• MDL Number: MFCD00009106
• SMILES: CCOC(=O)OC(=O)OCC

Computed Properties

• Complexity: 126
• Covalently-Bonded Unit Count: 1
• Heavy Atom Count: 11
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 6
• XLogP3: 1.4

Upstream Synthesis Route

Diethyl pyrocarbonate, commonly known as DEPC, is a versatile compound widely used in chemical synthesis for various applications. DEPC is primarily utilized as a reagent for the protection of amino groups in organic compounds. Its ability to modify nucleophilic functional groups makes it a valuable tool in the synthesis of pharmaceuticals, agrochemicals, and specialty chemicals. Additionally, DEPC is often employed as a reagent for the modification of nucleic acids, particularly for the protection of RNA against degradation by RNase enzymes. Its selective reactivity and compatibility with a wide range of organic compounds make DEPC a vital component in the toolbox of synthetic chemists.

Literature

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• Experimental infection of Balb/c nude mice with Hepatitis E virus.

  BMC infectious diseases 20090101

• Maternal epileptic seizure induced by pentylenetetrazol: apoptotic neurodegeneration and decreased GABAB1 receptor expression in prenatal rat brain.

  Molecular brain 20090101

• Primary Structure Revision and Active Site Mapping of E. Coli Isoleucyl-tRNA Synthetase by Means of Maldi Mass Spectrometry.

  The open biochemistry journal 20090101

• Evidence for oxidative stress in the developing cerebellum of the rat after chronic mild carbon monoxide exposure (0.0025% in air).

  BMC neuroscience 20090101

• Viral inactivation based on inhibition of membrane fusion: understanding the role of histidine protonation to develop new viral vaccines.

  Protein and peptide letters 20090101

• Eumycetozoa = Amoebozoa?: SSUrDNA phylogeny of protosteloid slime molds and its significance for the amoebozoan supergroup.

  PloS one 20090101

• Laser capture microdissection of gonads from juvenile zebrafish.

  Reproductive biology and endocrinology : RB&E 20090101

• Exon expression in lymphoblastoid cell lines from subjects with schizophrenia before and after glucose deprivation.

  BMC medical genomics 20090101

• Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses.

  BMC research notes 20090101

• Simultaneous transcription of duplicated var2csa gene copies in individual Plasmodium falciparum parasites.

  Genome biology 20090101

• PD 0332991, a selective cyclin D kinase 4/6 inhibitor, preferentially inhibits proliferation of luminal estrogen receptor-positive human breast cancer cell lines in vitro.

  Breast cancer research : BCR 20090101

• Purification and characterization of cysteine protease from germinating cotyledons of horse gram.

  BMC biochemistry 20090101

• Vasospastic individuals demonstrate significant similarity to glaucoma patients as revealed by gene expression profiling in circulating leukocytes.

  Molecular vision 20090101

• Multiple recombinants in two dengue virus, serotype-2 isolates from patients from Oaxaca, Mexico.

  BMC microbiology 20090101

• The effect of calcipotriol on the expression of human beta defensin-2 and LL-37 in cultured human keratinocytes.

  Clinical & developmental immunology 20090101

• Native R-loops persist throughout the mouse mitochondrial DNA genome.

  The Journal of biological chemistry 20081226

• Thymine dimer-induced structural changes to the DNA duplex examined with reactive probes (†).

  Biochemistry 20081209

• CsPLDalpha1 and CsPLDgamma1 are differentially induced during leaf and fruit abscission and diurnally regulated in Citrus sinensis.

  Journal of experimental botany 20081001

• Overexpression of USF increases TGF-beta1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells.

  Journal of Korean medical science 20081001

• Isolation and characterization of superoxide dismutase from wheat seedlings.

  Journal of agricultural and food chemistry 20080910

• Identification of the copper(II) coordinating residues in the prion protein by metal-catalyzed oxidation mass spectrometry: evidence for multiple isomers at low copper(II) loadings.

  Biochemistry 20080902

• A small stem loop element directs internal initiation of the URE2 internal ribosome entry site in Saccharomyces cerevisiae.

  The Journal of biological chemistry 20080704

• Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat).

  Bioscience, biotechnology, and biochemistry 20080601

• Characterization of heme-coordinating histidyl residues of an engineered six-coordinated myoglobin mutant based on the reactivity with diethylpyrocarbonate, mass spectrometry, and electron paramagnetic resonance spectroscopy.

  Journal of bioscience and bioengineering 20080601

• Reactivity of histidine and lysine side-chains with diethylpyrocarbonate -- a method to identify surface exposed residues in proteins.

  Journal of biochemical and biophysical methods 20080424

• Protein surface mapping using diethylpyrocarbonate with mass spectrometric detection.

  Analytical chemistry 20080415

• Evaluation of the inhibitory effect of dimethyl dicarbonate (DMDC) against wine microorganisms.

  Food microbiology 20080401

• Structure-function studies on the active site of the coelenterazine-dependent luciferase from Renilla.

  Protein science : a publication of the Protein Society 20080401

• Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient.

  Journal of virological methods 20080201

• Site-directed mutagenesis of Arginine282 suggests how protons and peptides are co-transported by rabbit PepT1.

  The international journal of biochemistry & cell biology 20080101

• Expression of RHOGTPase regulators in human myometrium.

  Reproductive biology and endocrinology : RB&E 20080101

• Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis.

  BMC plant biology 20080101

• Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses.

  Virology journal 20080101

• Brain neuronal CB2 cannabinoid receptors in drug abuse and depression: from mice to human subjects.

  PloS one 20080101

• Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis.

  Molecular vision 20080101

• Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species.

  BMC genomics 20080101

• Whole tumor antigen vaccination using dendritic cells: comparison of RNA electroporation and pulsing with UV-irradiated tumor cells.

  Journal of translational medicine 20080101

• Gene discovery in an invasive tephritid model pest species, the Mediterranean fruit fly, Ceratitis capitata.

  BMC genomics 20080101

• Enhancer of the rudimentary gene homologue (ERH) expression pattern in sporadic human breast cancer and normal breast tissue.

  BMC cancer 20080101

• Intestinal colonization of IL-2 deficient mice with non-colitogenic B. vulgatus prevents DC maturation and T-cell polarization.

  PloS one 20080101

• Identification of a set of genes showing regionally enriched expression in the mouse brain.

  BMC neuroscience 20080101

• Specific genetic markers for detecting subtypes of dengue virus serotype-2 in isolates from the states of Oaxaca and Veracruz, Mexico.

  BMC microbiology 20080101

• Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays.

  BMC genomics 20080101

• The -786T>C promoter polymorphism of the NOS3 gene is associated with prostate cancer progression.

  BMC cancer 20080101

• [Identification of catalytically active groups of wheat (Triticum aestivum L.) germ lipase].

  Prikladnaia biokhimiia i mikrobiologiia 20080101

• Evidence that molecular changes in cells occur before morphological alterations during the progression of breast ductal carcinoma.

  Breast cancer research : BCR 20080101

• Bridging the synaptic gap: neuroligins and neurexin I in Apis mellifera.

  PloS one 20080101

• Sumatriptan inhibits synaptic transmission in the rat midbrain periaqueductal grey.

  Molecular pain 20080101

• Molecular characterisation of a bovine-like rotavirus detected from a giraffe.

  BMC veterinary research 20080101

• Differential neuroglycan C expression during retinal degeneration in Rpe65-/- mice.

  Molecular vision 20080101

• MFS transportome of the human pathogenic yeast Candida albicans.

  BMC genomics 20080101

• Increased expression of lipocalin-type prostaglandin D2 synthase in osteoarthritic cartilage.

  Arthritis research & therapy 20080101

• Mir-434-5p mediates skin whitening and lightening.

  Clinical, cosmetic and investigational dermatology 20080101

• In vitro irradiation of basement membrane enhances the invasiveness of breast cancer cells.

  British journal of cancer 20071203

• New chemical method of viral inactivation for vaccine development based on membrane fusion inhibition.

  Vaccine 20071114

• Copper sensing based on the far-red fluorescent protein, HcRed, from Heteractis crispa.

  Analytical biochemistry 20071101

• Preparing glass slides and coverslips for in situ hybridization.

  CSH protocols 20071101

• A role for His155 in binding of human prion peptide144-167 to immobilised prion protein.

  Biochemical and biophysical research communications 20071026

• Substrate analysis of homoserine acyltransferase from Bacillus cereus.

  Biochemical and biophysical research communications 20070921

• Purification and characterization of a new endoglucanase from Aspergillus aculeatus.

  Journal of agricultural and food chemistry 20070905

• Flip-flop of fluorescently labeled phospholipids in proteoliposomes reconstituted with Saccharomyces cerevisiae microsomal proteins.

  Eukaryotic cell 20070901

• Downregulation of the RUNX3 gene by promoter hypermethylation and hemizygous deletion in breast cancer.

  Journal of Korean medical science 20070901

• Effect of divalent heavy metals on epithelial Na+ channels in A6 cells.

  American journal of physiology. Renal physiology 20070701

• Probing the promiscuous active site of myo-inositol dehydrogenase using synthetic substrates, homology modeling, and active site modification.

  Biochemistry 20070626

• Chromosome 9p21 gene copy number and prognostic significance of p16 in ESFT.

  British journal of cancer 20070618

• Identification of essential histidine and cysteine residues of the H+/organic cation antiporter multidrug and toxin extrusion (MATE).

  Molecular pharmacology 20070601

• Tickborne encephalitis in naturally exposed monkey (Macaca sylvanus).

  Emerging infectious diseases 20070601

• Preferential intramolecular ring closure of aminoalcohols with diethyl carbonate to oxazolidinones.

  Chemical & pharmaceutical bulletin 20070501

• The Anti-Obesity Effect of the Palatinose-Based Formula Inslow is Likely due to an Increase in the Hepatic PPAR-alpha and Adipocyte PPAR-gamma Gene Expressions.

  Journal of clinical biochemistry and nutrition 20070501

• Binding of a single zinc ion to one subunit of copper-zinc superoxide dismutase apoprotein substantially influences the structure and stability of the entire homodimeric protein.

  Journal of the American Chemical Society 20070418

• Characterization and copper binding properties of human COMMD1 (MURR1).

  Biochemistry 20070320

• A base-catalyzed mechanism for dark state recovery in the Avena sativa phototropin-1 LOV2 domain.

  Biochemistry 20070320

• Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima.

  Applied microbiology and biotechnology 20070301

• Biochemical analysis of ecto-nucleotide pyrophosphatase phosphodiesterase activity in brain membranes indicates involvement of NPP1 isoenzyme in extracellular hydrolysis of diadenosine polyphosphates in central nervous system.

  Neurochemistry international 20070301

• Impact of trivalent arsenicals on selenoprotein synthesis.

  Environmental health perspectives 20070301

• Transmembrane domain histidines contribute to regulation of AE2-mediated anion exchange by pH.

  American journal of physiology. Cell physiology 20070201

• A suite of sucrose transporters expressed in coats of developing legume seeds includes novel pH-independent facilitators.

  The Plant journal : for cell and molecular biology 20070201

• Inactivation of vesicular stomatitis virus through inhibition of membrane fusion by chemical modification of the viral glycoprotein.

  Antiviral research 20070101

• microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder.

  Genome biology 20070101

• Purification and some properties of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans.

  Preparative biochemistry & biotechnology 20070101

• Abundant L-type calcium channel Ca(v)1.3 (alpha1D) subunit mRNA is detected in rod photoreceptors of the mouse retina via in situ hybridization.

  Molecular vision 20070101

• Inhibition of advanced glycation and absence of galectin-3 prevent blood-retinal barrier dysfunction during short-term diabetes.

  Experimental diabetes research 20070101

• A transcriptomic analysis of the adult stage of the bovine lungworm, Dictyocaulus viviparus.

  BMC genomics 20070101

• The expression of TIMPs in cryo-preserved and freeze-dried amniotic membrane.

  Current eye research 20070101

• Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes.

  Arthritis research & therapy 20070101

• Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa.

  Genome biology 20070101

• Comparative evaluation of antiproliferative, antiangiogenic and apoptosis inducing potential of black tea polyphenols in the hamster buccal pouch carcinogenesis model.

  Journal of carcinogenesis 20070101

• G and P genotyping of human rotavirus isolated in a university hospital in Korea: implications for nosocomial infections.

  Journal of Korean medical science 20061201

• Proteins in load-bearing junctions: the histidine-rich metal-binding protein of mussel byssus.

  Biochemistry 20061128

• Combination of NADPH and copper ions generates proteinase K-resistant aggregates from recombinant prion protein.

  The Journal of biological chemistry 20061117

• A novel regulatory metal binding domain is present in the C terminus of Arabidopsis Zn2+-ATPase HMA2.

  The Journal of biological chemistry 20061110

• Catalytic characteristics of peroxidase from wheat grass.

  Journal of agricultural and food chemistry 20061101

• Genotoxicants target distinct molecular networks in neonatal neurons.

  Environmental health perspectives 20061101

• Identification of oxidized methionine sites in erythrocyte membrane protein by liquid chromatography/electrospray ionization mass spectrometry peptide mapping.

  Biochemistry 20061003

• Characterization of heme-coordinating histidyl residues of cytochrome b5 based on the reactivity with diethylpyrocarbonate: a mechanism for the opening of axial imidazole rings.

  Journal of biochemistry 20061001

• The role of the histidine-35 residue in the cytocidal action of HM-1 killer toxin.

  Microbiology (Reading, England) 20061001

• Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX.

  The Journal of biological chemistry 20060818

• Nucleoside diphosphate kinase A as a controller of AMP-kinase in airway epithelia.

  Journal of bioenergetics and biomembranes 20060801

• Influence of a proton gradient on the transport kinetics of the H+/amino acid cotransporter PAT1 in Caco-2 cells.

  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 20060701

• A mass spectrometric investigation of the binding of gold antiarthritic agents and the metabolite [Au(CN)2]- to human serum albumin.

  Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 20060701

• Uncoupling of ATP-mediated calcium signaling and dysregulated interleukin-6 secretion in dendritic cells by nanomolar thimerosal.

  Environmental health perspectives 20060701

• The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic and computational modeling analyses.

  Purinergic signalling 20060601

• Efficient suppression of secretory clusterin levels by polymer-siRNA nanocomplexes enhances ionizing radiation lethality in human MCF-7 breast cancer cells in vitro.

  International journal of nanomedicine 20060601

• Biochemical and immunochemical characterisation of human diadenosine triphosphatase provides evidence for its identification with the tumour suppressor Fhit protein.

  Biochimie 20060501

• The extracellular pH dependency of transport activity by human oligopeptide transporter 1 (hPEPT1) expressed stably in Chinese hamster ovary (CHO) cells: a reason for the bell-shaped activity versus pH.

  Biological & pharmaceutical bulletin 20060501

• Broad-spectrum antioxidant peptides derived from His residue-containing sequences present in human paraoxonase 1.

  Free radical research 20060401

• Functional contribution of a conserved, mobile loop histidine of phosphoribulokinase.

  Protein science : a publication of the Protein Society 20060401

• Imidazole binding reagent diethyl pyrocarbonate (DEPC) inhibits avian intrapulmonary chemoreceptor discharge in vivo.

  Respiratory physiology & neurobiology 20060227

• Diethyl pyrocarbonate, a histidine-modifying agent, directly stimulates activity of ATP-sensitive potassium channels in pituitary GH(3) cells.

  Biochemical pharmacology 20060227

• Optimum activity of the phosphofructokinase from Ascaris suum requires more than one metal ion.

  Biochemistry 20060221

• Characterization of a sialidase (neuraminidase) isolated from Clostridium chauvoei (Jakari strain).

  Cell biochemistry and function 20060101

• Modulation of inhibitory glycine receptors in cultured embryonic mouse hippocampal neurons by zinc, thiol containing redox agents and carnosine.

  Neuroscience 20060101

• Spontaneous rescue from cystic fibrosis in a mouse model.

  BMC genetics 20060101

• Measuring nasal bacterial load and its association with otitis media.

  BMC ear, nose, and throat disorders 20060101

• Characterization of the ribonuclease activity on the skin surface.

  Genetic vaccines and therapy 20060101

• Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival.

  BMC cancer 20060101

• PI3K signaling and miRNA expression during the response of quiescent human fibroblasts to distinct proliferative stimuli.

  Genome biology 20060101

• The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.

  BMC microbiology 20060101

• Kinetics of inactivation of phytase (phy A) during modification of histidine residue by IAA and DEP.

  Protein and peptide letters 20060101

• Expression patterns of plexins and neuropilins are consistent with cooperative and separate functions during neural development.

  BMC developmental biology 20060101

• Latest developments in in situ PCR.

  Methods in molecular biology (Clifton, N.J.) 20060101

• Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability.

  BMC plant biology 20060101

• Sex-specific expression of alternative transcripts in Drosophila.

  Genome biology 20060101

• Green fluorescent protein as a reporter of prion protein folding.

  Virology journal 20060101

• Effects of THBS3, SPARC and SPP1 expression on biological behavior and survival in patients with osteosarcoma.

  BMC cancer 20060101

• Studies on the CPA cysteine peptidase in the Leishmania infantum genome strain JPCM5.

  BMC molecular biology 20060101

• An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development.

  BMC plant biology 20060101

• Effects of in vitro fertilization and embryo culture on TRP53 and Bax expression in B6 mouse embryos.

  Reproductive biology and endocrinology : RB&E 20060101

• The Rv0805 gene from Mycobacterium tuberculosis encodes a 3',5'-cyclic nucleotide phosphodiesterase: biochemical and mutational analysis.

  Biochemistry 20051206

• Binding residues and catalytic domain of soluble Saccharomyces cerevisiae processing alpha-glucosidase I.

  Glycobiology 20051201

• Probing the role of the hyper-reactive histidine residue of arginase.

  Archives of biochemistry and biophysics 20051201

• Polyhistidine tract expansions in HOXA1 result in intranuclear aggregation and increased cell death.

  Biochemical and biophysical research communications 20051104

• Mutating His29, His125, His133 or His158 abolishes glycosylphosphatidylinositol-specific phospholipase D catalytic activity.

  The Biochemical journal 20051015

• pH-Dependent interaction of cytochrome c with mitochondrial mimetic membranes: the role of an array of positively charged amino acids.

  The Journal of biological chemistry 20051014

• Kinetic comparison of procaspase-3 and caspase-3.

  Archives of biochemistry and biophysics 20051001

• Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer.

  British journal of cancer 20050919

• Heparin-binding sites in granulocyte-macrophage colony-stimulating factor. Localization and regulation by histidine ionization.

  The Journal of biological chemistry 20050909

• Neonicotinoid nitroguanidine insecticide metabolites: synthesis and nicotinic receptor potency of guanidines, aminoguanidines, and their derivatives.

  Chemical research in toxicology 20050901

• Purification and characterization of a serine alkaline protease from Bacillus clausii GMBAE 42.

  Journal of industrial microbiology & biotechnology 20050801

• Direct Yellow and Methylene Blue liquid-liquid extraction with alkylene carbonates.

  Chemosphere 20050801

• Amino acid residues associated with enzymatic activities of the isomerizing phycoviolobilin-lyase PecE/F.

  Biochemistry 20050607

• The effect of simvastatin on the proliferation and differentiation of human bone marrow stromal cells.

  Journal of Korean medical science 20050601

• Mechanism of horseradish peroxidase-catalyzed heme oxidation and polymerization (beta-hematin formation).

  Biochimica et biophysica acta 20050525

• [Pyrocarbon prosthesis for finger interphalangeal joint replacement. Experience after one year].

  Der Unfallchirurg 20050501

• Site-directed mutagenesis of active site residues of phosphite dehydrogenase.

  Biochemistry 20050329

• Two SUR1-specific histidine residues mandatory for zinc-induced activation of the rat KATP channel.

  The Journal of biological chemistry 20050311

• Spacer-based selectivity in the binding of 'two-prong' ligands to recombinant human carbonic anhydrase I.

  Biochemistry 20050308

• Effect of chemical modification of histidines on the copper-induced oligomerization of jack bean urease (EC 3.5.1.5).

  Archives of biochemistry and biophysics 20050301

• Rapid identification of emerging pathogens: coronavirus.

  Emerging infectious diseases 20050301

• Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii beta-glucosidase II with high hydrolytic activity on sophorose.

  Applied microbiology and biotechnology 20050201

• A possible site of superoxide generation in the complex I segment of rat heart mitochondria.

  Journal of bioenergetics and biomembranes 20050201

• Identification of an ascorbate-dependent cytochrome b of the tonoplast membrane sharing biochemical features with members of the cytochrome b561 family.

  Planta 20050101

• Purification and characterization of an esterase hydrolyzing monoalkyl phthalates from Micrococcus sp. YGJ1.

  Journal of biochemistry 20050101

• L-histidine inhibits production of lysophosphatidic acid by the tumor-associated cytokine, autotaxin.

  Lipids in health and disease 20050101

• Alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome P450 encoded on the nifD excision element of Anabaena 7120.

  BMC microbiology 20050101

• Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR.

  BMC molecular biology 20050101

• Creatine synthesis and transport during rat embryogenesis: spatiotemporal expression of AGAT, GAMT and CT1.

  BMC developmental biology 20050101

• Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.

  Cell communication and signaling : CCS 20050101

• Genomic responses in rat cerebral cortex after traumatic brain injury.

  BMC neuroscience 20050101

• Differential expression of TGF-beta isoforms during differentiation of HaCaT human keratinocyte cells: implication for the separate role in epidermal differentiation.

  Journal of Korean medical science 20041201

• Mutational analysis of histidine residues in human organic anion transporter 4 (hOAT4).

  The Biochemical journal 20041115

• Biochemical characterization of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphorybosyltransferase: role of histidine residue in substrate selectivity.

  Molecular and biochemical parasitology 20041001

• The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae.

  Insect biochemistry and molecular biology 20041001

• Sequence selective binding of bis-daunorubicin WP631 to DNA.

  European journal of biochemistry 20040901

• Architecture of a Diels-Alderase ribozyme with a preformed catalytic pocket.

  Chemistry & biology 20040901

• Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER.

  Biochemistry 20040824

• Enzymatic synthesis and chemical recycling of poly(carbonate-urethane).

  Macromolecular bioscience 20040809

• Molecular basis for modulation of recombinant alpha1beta2gamma2 GABAA receptors by protons.

  Journal of neurophysiology 20040801

• The renal-specific transporter mediates facilitative transport of organic anions at the brush border membrane of mouse renal tubules.

  Journal of the American Society of Nephrology : JASN 20040801

• Expression of cyclodextrinase gene from Paenibacillus sp. A11 in Escherichia coli and characterization of the purified cyclodextrinase.

  Journal of biochemistry and molecular biology 20040731

• Inhibition by copper(II) binding of hepatocyte growth factor (HGF) interaction with its receptor Met and blockade of HGF/Met function.

  The Journal of biological chemistry 20040730

• Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties.

  The international journal of biochemistry & cell biology 20040701

• Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12.

  Biochemistry 20040622

• Identification and synthesis of a novel selenium-sulfur amino acid found in selenized yeast: Rapid indirect detection NMR methods for characterizing low-level organoselenium compounds in complex matrices.

  Journal of agricultural and food chemistry 20040616

• Functional reconstitution into proteoliposomes and partial purification of a rat liver ER transport system for a water-soluble analogue of mannosylphosphoryldolichol.

  Biochemistry 20040615

• Inhibition studies on Rhodospirillum rubrum H(+)-pyrophosphatase expressed in Escherichia coli.

  Biochimica et biophysica acta 20040607

• Neonatal Fc receptors discriminates and monitors the pathway of native and modified immunoglobulin G in placental endothelial cells.

  Human immunology 20040601

• Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana. Histidines that coordinate the two metal ions in the active site show different susceptibilities to irreversible chemical modification.

  European journal of biochemistry 20040501

• Thermodynamic studies of the mechanism of metal binding to the Escherichia coli zinc transporter YiiP.

  The Journal of biological chemistry 20040423

• Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase.

  Biochemistry 20040420

• Histidine residues in human phenol sulfotransferases.

  Biochemical pharmacology 20040401

• Ff gene 5 single-stranded DNA-binding protein assembles on nucleotides constrained by a DNA hairpin.

  Biochemistry 20040309

• pH dependence of the efficiency of binding of iron cations to the donor side of photosystem II.

  Biochemistry. Biokhimiia 20040301

• [Product analysis of oxo synthesis of diethyl carbonate by GC/MS].

  Se pu = Chinese journal of chromatography 20040301

• Roles of histidine residues in plant vacuolar H(+)-pyrophosphatase.

  Biochimica et biophysica acta 20040215

• Constitutive activation and uncoupling of the atrial natriuretic peptide receptor by mutations at the dimer interface. Role of the dimer structure in signalling.

  The Journal of biological chemistry 20040213

• Purification and characterization of ascorbic acid 2-kinase from Flavobacterium devorans ATCC 10829.

  Biochimie 20040201

• Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells.

  Indian journal of biochemistry & biophysics 20040201

• Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver.

  Archives of biochemistry and biophysics 20040101

• Properties of two distinct heme centers of cytochrome b561 from bovine chromaffin vesicles studied by EPR, resonance Raman, and ascorbate reduction assay.

  Journal of biochemistry 20040101

• Human glutaminyl cyclase and bacterial zinc aminopeptidase share a common fold and active site.

  BMC biology 20040101

• Genomic structure and alternative splicing of murine R2B receptor protein tyrosine phosphatases (PTPkappa, mu, rho and PCP-2).

  BMC genomics 20040101

• P2 receptor mRNA expression profiles in human lymphocytes, monocytes and CD34+ stem and progenitor cells.

  BMC immunology 20040101

• Gene expression variation between mouse inbred strains.

  BMC genomics 20040101

• RNA integrity as a quality indicator during the first steps of RNP purifications : a comparison of yeast lysis methods.

  BMC biochemistry 20040101

• Divergence of the mRNA targets for the Ssb proteins of bacteriophages T4 and RB69.

  Virology journal 20040101

• Chloroplast biogenesis 88. Protochlorophyllide b occurs in green but not in etiolated plants.

  The Journal of biological chemistry 20031212

• Characterization of sterol uptake in leaf tissues of sugar beet.

  Planta 20031201

• Inhibition of Escherichia coli RNase P by oligonucleotide directed misfolding of RNA.

  RNA (New York, N.Y.) 20031201

• Reversely-oriented cytochrome b561 in reconstituted vesicles catalyzes transmembrane electron transfer and supports extravesicular dopamine beta-hydroxylase activity.

  Journal of biochemistry 20031201

• Histidine-834 of human erythrocyte band 3 has an essential role in the conformational changes that occur during the band 3-mediated anion exchange.

  Biochemistry 20031111

• The structural and functional studies of His119 and His12 in RNase A via chemical modification.

  Journal of protein chemistry 20031101

• Chemical modification of contractile 3-nm-diameter filaments in Vorticella spasmoneme by diethyl-pyrocarbonate and its reversible renaturation by hydroxylamine.

  Biochemical and biophysical research communications 20031031

• Critical role of histidine residues in cyclohexanone monooxygenase expression, cofactor binding and catalysis.

  Chemico-biological interactions 20031025

• Mass-production of human ACAT-1 and ACAT-2 to screen isoform-specific inhibitor: a different substrate specificity and inhibitory regulation.

  Biochemical and biophysical research communications 20031003

• Rat kidney acylase I: further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process.

  Biochimie 20031001

• Binding of the human 'electron transferring flavoprotein' (ETF) to the medium chain acyl-CoA dehydrogenase (MCAD) involves an arginine and histidine residue.

  Journal of enzyme inhibition and medicinal chemistry 20031001

• Sodium influx and accumulation in Arabidopsis.

  Plant physiology 20030901

• [Arthroplasty of the metacarpophalangeal joint using pyrocarbonate implants].

  Der Orthopade 20030901

• Chemical modification of catalytically essential functional groups of NAD-dependent hydrogenase from Ralstonia eutropha H16.

  Biochemistry. Biokhimiia 20030901

• Identification of essential histidines in cyclodextrin glycosyltransferase isoform 1 from Paenibacillus sp. A11.

  Journal of biochemistry and molecular biology 20030731

• Stopped-flow analyses on the reaction of ascorbate with cytochrome b561 purified from bovine chromaffin vesicle membranes.

  Biochemistry 20030715

• His1205 and His1223 are essential for the activity of the mitogenic Pasteurella multocida toxin.

  Biochemistry 20030506

• Stimulatory action of internal protons on Slo1 BK channels.

  Biophysical journal 20030501

• Membrane fusion induced by vesicular stomatitis virus depends on histidine protonation.

  The Journal of biological chemistry 20030418

• Purification and characterization of PrbA, a new esterase from Enterobacter cloacae hydrolyzing the esters of 4-hydroxybenzoic acid (parabens).

  The Journal of biological chemistry 20030411

• Chemical modification of microcin J25 with diethylpyrocarbonate and carbodiimide: evidence for essential histidyl and carboxyl residues.

  Biochemical and biophysical research communications 20030404

• Expression and characterization of human bifunctional peptidylglycine alpha-amidating monooxygenase.

  Protein expression and purification 20030401

• Impact of purified water quality on molecular biology experiments.

  Clinical chemistry and laboratory medicine 20030401

• Citrate uniport by the mitochondrial tricarboxylate carrier: a basis for a new hypothesis for the transport mechanism.

  Journal of bioenergetics and biomembranes 20030401

• The PrP-like protein Doppel binds copper.

  The Journal of biological chemistry 20030314

• Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta.

  The Journal of biological chemistry 20030227

• Identification of the essential histidine residue for high-affinity binding of AlbA protein to albicidin antibiotics.

  Microbiology (Reading, England) 20030201

• Transcellular transport of a highly polar 3+ net charge opioid tetrapeptide.

  The Journal of pharmacology and experimental therapeutics 20030101

• Potent P2Y6 receptor mediated contractions in human cerebral arteries.

  BMC pharmacology 20030101

• Chemical modifications of alpha1,6-fucosyltransferase define amino acid residues of catalytic importance.

  Biochimie 20030101

• Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

  Acta biochimica Polonica 20030101

• Decreased expression of beta1- and beta2-adrenoceptors in human diabetic atrial appendage.

  Cardiovascular diabetology 20030101

• Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project.

  BMC genomics 20030101

• Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues.

  Molecular cancer 20030101

• External nickel inhibits epithelial sodium channel by binding to histidine residues within the extracellular domains of alpha and gamma subunits and reducing channel open probability.

  The Journal of biological chemistry 20021220

• Identification of a key residue in the conformational stability of acyl carrier protein.

  Biochimica et biophysica acta 20021216

• Arabidopsis thaliana root non-selective cation channels mediate calcium uptake and are involved in growth.

  The Plant journal : for cell and molecular biology 20021201

• p-Aminohippurate transport at the apical membrane in the OK kidney epithelial cell line.

  Pharmaceutical research 20021201

• Biochemical characterization of the Pseudomonas putida 3-hydroxyacyl ACP:CoA transacylase, which diverts intermediates of fatty acid de novo biosynthesis.

  The Journal of biological chemistry 20021108

• N-terminal sequence and distal histidine residues are responsible for pH-regulated cytoplasmic membrane binding of human AMP deaminase isoform E.

  The Journal of biological chemistry 20021108

• survivin messenger RNA expression is a good prognostic biomarker for oesophageal carcinoma.

  British journal of cancer 20021007

• Fermentability of grape must after inhibition with dimethyl dicarbonate (DMDC).

  Journal of agricultural and food chemistry 20020925

• Different mechanisms underlying the stimulation of K(Ca) channels by nitric oxide and carbon monoxide.

  The Journal of clinical investigation 20020901

• A model for co-expression pattern analysis of genes implicated in angiogenesis and tumour cell invasion in cervical cancer.

  British journal of cancer 20020827

• Ion-molecule interactions in solutions of lithium perchlorate in propylene carbonate + diethyl carbonate mixtures: an IR and molecular orbital study.

  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 20020801

• Probing the role of active site histidine residues in the catalytic activity of lacrimal gland peroxidase.

  Molecular and cellular biochemistry 20020801

• Insights into the interaction of human liver arginase with tightly and weakly bound manganese ions by chemical modification and site-directed mutagenesis studies.

  Archives of biochemistry and biophysics 20020715

• Interaction of adenosine deaminase with inhibitors. Chemical modification by diethyl pyrocarbonate.

  Biochemistry. Biokhimiia 20020701

• Differential interactions of estrogens and antiestrogens at the 17 beta-hydroxyl or counterpart hydroxyl with histidine 524 of the human estrogen receptor alpha.

  Biochemistry 20020625

• A RAC protein-binding site in the internal transcribed spacer 2 of Pre-rRNA transcripts from Schizosaccharomyces pombe.

  The Journal of biological chemistry 20020614

• Hemin-stimulated docking of cytochrome c to a hemin-DNA aptamer complex.

  Biochemistry 20020423

• Hemichannel and junctional properties of connexin 50.

  Biophysical journal 20020401

• Overexpression, purification, and partial characterization of Saccharomyces cerevisiae processing alpha glucosidase I.

  Glycobiology 20020301

• Expression of uncoupling proteins-1, -2 and -3 mRNA is induced by an adenocarcinoma-derived lipid-mobilizing factor.

  British journal of cancer 20020212

• The peroxisome proliferator-induced cytosolic type I acyl-CoA thioesterase (CTE-I) is a serine-histidine-aspartic acid alpha /beta hydrolase.

  The Journal of biological chemistry 20020201

• Molecular characteristics of small intestinal and renal brush border thiamin transporters in rats.

  Biochimica et biophysica acta 20020201

• A pH-dependent conformational transition of Abeta peptide and physicochemical properties of the conformers in the glial cell.

  The Biochemical journal 20020201

• Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots.

  Plant physiology 20020201

• Chemical modification of catalytic site of lipase from wheat germ: altered structure-activity profile.

  Indian journal of biochemistry & biophysics 20020201

• Identification of histidine residues at the active site of Megalobatrachus japonicus alkaline phosphatase by chemical modification.

  Biochimica et biophysica acta 20020131

• Mapping Cu(II) binding sites in prion proteins by diethyl pyrocarbonate modification and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometric footprinting.

  The Journal of biological chemistry 20020118

• cDNA cloning, biological and immunological characterization of the alkaline serine protease major allergen from Penicillium chrysogenum.

  International archives of allergy and immunology 20020101

• Diethylpyrocarbonate inhibition of vacuolar H+-pyrophosphatase possibly involves a histidine residue.

  Journal of protein chemistry 20020101

• Multi site polyadenylation and transcriptional response to stress of a vacuolar type H+-ATPase subunit A gene in Arabidopsis thaliana.

  BMC plant biology 20020101

• Regional differences in expression of VEGF mRNA in rat gastrocnemius following 1 hr exercise or electrical stimulation.

  BMC physiology 20020101

• Regulation of Na+/H+ exchanger by urogastrone, a potent activator of cell proliferation.

  Physiological research 20020101

• Possible involvement of a single histidine residue in the P-type Na+-ATPase of a facultatively anaerobic alkaliphile, Exiguobacterium aurantiacum.

  Current microbiology 20011201

• Effects of the position of internal histidine residues on the collision-induced fragmentation of triply protonated tryptic peptides.

  Journal of the American Society for Mass Spectrometry 20011201

• Structure-function analysis of the ArsA ATPase: contribution of histidine residues.

  Journal of bioenergetics and biomembranes 20011201

• Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

  FEBS letters 20011123

• Mechanism of ascorbic acid oxidation by cytochrome b(561).

  Biochemistry 20011002

• Modular construction of a tertiary RNA structure: the specificity domain of the Bacillus subtilis RNase P RNA.

  Biochemistry 20010918

• Evidence for essential histidines in human pituitary glutaminyl cyclase.

  Biochemistry 20010918

• Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying agent: evidence for at least two target sites.

  The Biochemical journal 20010901

• Factors determining the special redox properties of photosynthetic cytochrome b559.

  European journal of biochemistry 20010901

• An essential histidine residue in GTP binding domain of bovine brain glutamate dehydrogenase isoproteins.

  Molecules and cells 20010831

• Heme histidine ligands within gp91(phox) modulate proton conduction by the phagocyte NADPH oxidase.

  The Journal of biological chemistry 20010810

• Conformational change in the vinculin C-terminal depends on a critical histidine residue (His-906).

  The Journal of biological chemistry 20010803

• Slowing of ERG current deactivation in NG108-15 cells by the histidine-specific reagent diethylpyrocarbonate.

  Neuropharmacology 20010801

• Functional residues on the enzyme active site of glyoxalase I from bovine brain.

  Preparative biochemistry & biotechnology 20010801

• [An IR study of ion solvation and association of lithium perchlorate in some organic solvents].

  Guang pu xue yu guang pu fen xi = Guang pu 20010801

• Chemical reagents for investigating the major groove of DNA.

  Current protocols in nucleic acid chemistry 20010801

• Involvement of the chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase sequence His444-Arg-Glu-Arg in modulation of the bisphosphatase activity by its kinase domain.

  The Biochemical journal 20010715

• Inhibition of plant vacuolar H(+)-ATPase by diethylpyrocarbonate.

  Biochimica et biophysica acta 20010702

• A common inhibitory binding site for zinc and odorants at the voltage-gated K(+) channel of rat olfactory receptor neurons.

  The European journal of neuroscience 20010701

• Mechanism and requirements for bovine papillomavirus, type 1, E1 initiator complex assembly promoted by the E2 transcription factor bound to distal sites.

  The Journal of biological chemistry 20010629

• Mg2+-induced tRNA folding.

  Biochemistry 20010605

• Heparin-binding histidine and lysine residues of rat selenoprotein P.

  The Journal of biological chemistry 20010511

• The importance of histidine residues in human ecto-nucleoside triphosphate diphosphohydrolase-3 as determined by site-directed mutagenesis.

  Biochimica et biophysica acta 20010505

• Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity.

  The Journal of biological chemistry 20010504

• Probing the binding site of 800-nm bacteriochlorophyll in the membrane-linked LH2 protein of Rhodobacter capsulatus by local unfolding and chemical modification: evidence for the involvement of a betaHis20 residue.

  European journal of biochemistry 20010501

• Conformation and polarity of the active site of xylanase I from Thermomonospora sp. as deduced by fluorescent chemoaffinity labeling. Site and significance of a histidine residue.

  European journal of biochemistry 20010501

• Preparation of bacterial RNA.

  Current protocols in molecular biology 20010501

• Probing RNA structure with chemical reagents and enzymes.

  Current protocols in nucleic acid chemistry 20010501

• Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) with the group I intron P4-P6 domain. Thermodynamic analysis and the role of metal ions.

  Journal of molecular biology 20010427

• Insights into the reaction mechanism of the diisopropyl fluorophosphatase from Loligo vulgaris by means of kinetic studies, chemical modification and site-directed mutagenesis.

  Biochimica et biophysica acta 20010407

• Evidence for an essential histidine residue in the ascorbate-binding site of cytochrome b561.

  Biochemistry 20010403

• Ascorbate inhibits the carbethoxylation of two histidyl and one tyrosyl residues indispensable for the transmembrane electron transfer reaction of cytochrome b561.

  Biochemistry 20010403

• Measurement of copper-binding sites on low density lipoprotein.

  Arteriosclerosis, thrombosis, and vascular biology 20010401

• In situ hybridization: use of 35S-labeled probes on paraffin tissue sections.

  Methods (San Diego, Calif.) 20010401

• Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase.

  Archives of biochemistry and biophysics 20010401

• Enzymatic active site of caspase-activated DNase (CAD) and its inhibition by inhibitor of CAD.

  Archives of biochemistry and biophysics 20010401

• Altering the intermediate in the equilibrium folding of unmodified yeast tRNAPhe with monovalent and divalent cations.

  Biochemistry 20010327

• Subunit-dependent modulation of neuronal nicotinic receptors by zinc.

  The Journal of neuroscience : the official journal of the Society for Neuroscience 20010315

• Tetraplex formation by the progressive myoclonus epilepsy type-1 repeat: implications for instability in the repeat expansion diseases.

  FEBS letters 20010302

• [The analysis of rice total RNA by capillary electrophoresis].

  Se pu = Chinese journal of chromatography 20010301

• Methane monooxygenase component B mutants alter the kinetics of steps throughout the catalytic cycle.

  Biochemistry 20010220

• Kinetic and mechanistic properties of biotin sulfoxide reductase.

  Biochemistry 20010206

• An essential role of active site arginine residue in iodide binding and histidine residue in electron transfer for iodide oxidation by horseradish peroxidase.

  Molecular and cellular biochemistry 20010201

• Characterization of yeast homoserine dehydrogenase, an antifungal target: the invariant histidine 309 is important for enzyme integrity.

  Biochimica et biophysica acta 20010112

• Modification of surface histidine residues abolishes the cytotoxic activity of Clostridium difficile toxin A.

  Toxicon : official journal of the International Society on Toxinology 20010101

• The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates.

  Journal of experimental botany 20010101

• The use of diethyl pyrocarbonate and potassium permanganate as probes for strand separation and structural distortions in DNA.

  Methods in molecular biology (Clifton, N.J.) 20010101

• High-resolution footprinting studies of drug-DNA complexes using chemical and enzymatic probes.

  Methods in enzymology 20010101

• RNA purification and quantification methods.

  Methods in molecular medicine 20010101

• Mechanisms of inhibitory effects of zinc and cadmium ions on agonist binding to adenosine A1 receptors in rat brain.

  Biochemical pharmacology 19990815

• Identification of the histidine residues involved in substrate recognition by a rat H+/peptide cotransporter, PEPT1.

  FEBS letters 19960930

• An intramolecular triplex in the human gamma-globin 5'-flanking region is altered by point mutations associated with hereditary persistence of fetal hemoglobin.

  The Journal of biological chemistry 19951013

• Active-site covalent modifications of quinoprotein amine oxidases from Aspergillus niger. Evidence for binding of the mechanism-based inhibitor, 1,4-diamino-2-butyne, to residue Lys356 involved in the catalytic cycle.

  European journal of biochemistry 19941101

• Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.

  Protein science : a publication of the Protein Society 19930601

• Chemical modification of bacteriophage T4 deoxynucleotide kinase. Evidence of a single catalytic region.

  The Journal of biological chemistry 19930125

• Differential effect of pH on thromboxane A2/prostaglandin H2 receptor agonist and antagonist binding in human platelets.

  The Journal of biological chemistry 19910725

• Role of basic amino acids in the interaction of bindin with sulfated fucans.

  Biochemistry 19881018

• Inhibition of oral carcinogenesis by a protease inhibitor.

  Journal of the National Cancer Institute 19860301

• Chemical modifications of Serratia marcescens anthranilate synthase component I.

  The Journal of biological chemistry 19811010

• Effect of diethylpyrocarbonate on the antiviral and interferon-inducing activities of viral and nonviral agents.

  Antimicrobial agents and chemotherapy 19760401