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Structural characterization and identification of major constituents in Jitai tablets by high-performance liquid chromatography/diode-array detection coupled with electrospray ionization tandem mass spectrometry.
Molecules (Basel, Switzerland) 20120903
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The cyanogenic syndrome in rubber tree Hevea brasiliensis: tissue-damage-dependent activation of linamarase and hydroxynitrile lyase accelerates hydrogen cyanide release.
Annals of botany 20120601
-
Gene cloning, expression, and characterization of a nitrilase from Alcaligenes faecalis ZJUTB10.
Journal of agricultural and food chemistry 20111109
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Cloning and expression of hydroxynitrile lyase gene from Eriobotrya japonica in Pichia pastoris.
Journal of bioscience and bioengineering 20111001
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Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants.
Protein engineering, design & selection : PEDS 20110801
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Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system.
Journal of biotechnology 20110310
-
Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(-)-mandelic acid production.
Bioprocess and biosystems engineering 20110301
-
Release of hydrogen cyanide via a post-secretion Schotten-Baumann reaction in defensive fluids of polydesmoid millipedes.
Journal of chemical ecology 20110301
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Enantioselective biocatalytic hydrolysis of (R,S)-mandelonitrile for production of (R)-(-)-mandelic acid by a newly isolated mutant strain.
Journal of industrial microbiology & biotechnology 20110201
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Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme.
Plant physiology 20110101
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Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid.
Microbial cell factories 20110101
-
Metabolites of amygdalin under simulated human digestive fluids.
International journal of food sciences and nutrition 20101201
-
Defensive secretions in three species of polydesmids (Diplopoda, Polydesmida, Polydesmidae).
Journal of chemical ecology 20100901
-
Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions.
Chemistry & biology 20100827
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Chiral solvating agents for cyanohydrins and carboxylic acids.
The Journal of organic chemistry 20100820
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Catalytic mechanism of hydroxynitrile lyase from Hevea brasiliensis: a theoretical investigation.
The journal of physical chemistry. B 20100729
-
Activity and enantioselectivity of the hydroxynitrile lyase MeHNL in dry organic solvents.
Chemistry (Weinheim an der Bergstrasse, Germany) 20100705
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Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401.
Journal of industrial microbiology & biotechnology 20100701
-
Bioproduction of glycolic acid from glycolonitrile with a new bacterial isolate of Alcaligenes sp. ECU0401.
Applied biochemistry and biotechnology 20100301
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Investigative mining of sequence data for novel enzymes: a case study with nitrilases.
Journal of biotechnology 20090810
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Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold.
Journal of biotechnology 20090520
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Dimethyl 1-(4-cyano-benz-yl)-1H-pyrazole-3,5-dicarboxyl-ate.
Acta crystallographica. Section E, Structure reports online 20090501
-
Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity.
Biochemistry 20090421
-
Enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies.
Molecular biotechnology 20090101
-
Utilization of arylaliphatic nitriles by haloalkaliphilic Halomonas nitrilicus sp. nov. isolated from soda soils.
Applied microbiology and biotechnology 20081101
-
Simultaneous expression of an arylacetonitrilase from Pseudomonas fluorescens and a (S)-oxynitrilase from Manihot esculenta in Pichia pastoris for the synthesis of (S)-mandelic acid.
Applied microbiology and biotechnology 20080801
-
Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina).
Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20080701
-
Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat).
Bioscience, biotechnology, and biochemistry 20080601
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Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor.
Bioscience, biotechnology, and biochemistry 20080601
-
3,3'-{1,1'-Methyl-enebis[naphthalene-2,1-diylbis(oxymethyl-ene)]}dibenzonitrile.
Acta crystallographica. Section E, Structure reports online 20080601
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4,4'-{[1,1'-Methyl-enebis(naphthalene-2,1-di-yl)]bis-(-oxymethyl-ene)}di-benzo-nitrile.
Acta crystallographica. Section E, Structure reports online 20080401
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Laboratory evolved biocatalysts for stereoselective syntheses of substituted benzaldehyde cyanohydrins.
Chembiochem : a European journal of chemical biology 20080104
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Organogenic nodule development in hop (Humulus lupulus L.): transcript and metabolic responses.
BMC genomics 20080101
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Characterisation of the substrate specificity of the nitrile hydrolyzing system of the acidotolerant black yeast Exophiala oligosperma R1.
Studies in mycology 20080101
-
Influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens EBC191.
Protein engineering, design & selection : PEDS 20070801
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Vicianin hydrolase is a novel cyanogenic beta-glycosidase specific to beta-vicianoside (6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside) in seeds of Vicia angustifolia.
Plant & cell physiology 20070701
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Discovery of a mandelonitrile hydrolase from Bradyrhizobium japonicum USDA110 by rational genome mining.
Journal of biotechnology 20070510
-
Serine scanning: a tool to prove the consequences of N-glycosylation of proteins.
Journal of biotechnology 20070330
-
Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis.
Journal of biotechnology 20070330
-
Stereoselective biocatalytic synthesis of (S)-2-hydroxy-2-methylbutyric acid via substrate engineering by using 'thio-disguised' precursors and oxynitrilase catalysis.
Chemistry (Weinheim an der Bergstrasse, Germany) 20070101
-
Tissue-specific mRNA expression profiling in grape berry tissues.
BMC genomics 20070101
-
Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.
Biotechnology letters 20061201
-
Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis.
Applied microbiology and biotechnology 20060801
-
A biocatalytic Henry reaction--the hydroxynitrile lyase from Hevea brasiliensis also catalyzes nitroaldol reactions.
Angewandte Chemie (International ed. in English) 20060519
-
Absorption of toxic beta-glucosides produced by plants and their effect on tissue trehalases from insects.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 20060301
-
Purification and characterization of an enantioselective arylacetonitrilase from Pseudomonas putida.
Archives of microbiology 20060201
-
The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection.
Journal of experimental botany 20060101
-
Screening for new hydroxynitrilases from plants.
Bioscience, biotechnology, and biochemistry 20051201
-
Male-fertility genes expressed in male flower buds of Silene latifolia include homologs of anther-specific genes.
Genes & genetic systems 20051201
-
Nitrilase from Pseudomonas fluorescens EBC191: cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme.
Microbiology (Reading, England) 20051101
-
Hydroxynitrile lyase catalysis in ionic liquid-containing systems.
Biotechnology letters 20050901
-
Cross-linked aggregates of (R)-oxynitrilase: a stable, recyclable biocatalyst for enantioselective hydrocyanation.
Organic letters 20050120
-
Synthesis of (R)-2-trimethylsilyl-2-hydroxyl-ethylcyanide catalyzed with (R)-oxynitrilase from loquat seed meal.
Biotechnology letters 20050101
-
Hydrolysis of nitriles using an immobilized nitrilase: applications to the synthesis of methionine hydroxy analogue derivatives.
Journal of agricultural and food chemistry 20041229
-
The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae.
Insect biochemistry and molecular biology 20041001
-
Cleaving of S-mandelonitrile catalyzed by S-hydroxynitrile lyase from Hevea brasiliensis--a kinetic investigation based on the rate curve method.
Journal of biotechnology 20040701
-
Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis.
Angewandte Chemie (International ed. in English) 20031013
-
A high-throughput amenable colorimetric assay for enantioselective screening of nitrilase-producing microorganisms using pH sensitive indicators.
Journal of biomolecular screening 20031001
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The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor.
Phytochemistry 20030901
-
Interfacial versus homogeneous enzymatic cleavage of mandelonitrile by hydroxynitrile lyase in a biphasic system.
Biotechnology and bioengineering 20030820
-
A single residual replacement improves the folding and stability of recombinant cassava hydroxynitrile lyase in E. coil.
Biotechnology letters 20030701
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Synthesis of degraded cyanogenic glycosides from Sambucus nigra.
Natural product research 20030601
-
Cyclooxygenase inhibitory and antioxidant compounds from crabapple fruits.
Journal of agricultural and food chemistry 20030326
-
(R)-oxynitrilase-catalysed synthesis of chiral silicon-containing aliphatic (R)-ketone-cyanohydrins.
Biotechnology letters 20030201
-
Purification and characterization of three beta-glycosidases from midgut of the sugar cane borer, Diatraea saccharalis.
Insect biochemistry and molecular biology 20030101
-
The toxicokinetics of cyanide and mandelonitrile in the horse and their relevance to the mare reproductive loss syndrome.
Toxicology mechanisms and methods 20030101
-
A simple and highly sensitive spectrophotometric method for the determination of cyanide in equine blood.
Toxicology mechanisms and methods 20030101
-
A practical high through-put continuous process for the synthesis of chiral cyanohydrins.
The Journal of organic chemistry 20021115
-
Investigation of the microheterogeneity and aglycone specificity-conferring residues of black cherry prunasin hydrolases.
Plant physiology 20020701
-
A kinetic model for enzyme interfacial activity and stability: pa-hydroxynitrile lyase at the diisopropyl ether/water interface.
Biotechnology and bioengineering 20020620
-
Lipases and (R)-oxynitrilases: useful tools in organic synthesis.
Journal of biotechnology 20020613
-
Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis.
European journal of biochemistry 20020601
-
Tertiary pentyl groups enhance salen titanium catalyst for highly enantioselective trimethylsilylcyanation of aldehydes.
The Journal of organic chemistry 20020419
-
Development of a process model to describe the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase in an aqueous-organic biphasic reactor.
Biotechnology and bioengineering 20020205
-
Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta.
Protein science : a publication of the Protein Society 20020101
-
Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants.
BMC plant biology 20020101
-
Biocatalytic synthesis of hydroxylated natural products using aldolases and related enzymes.
Current opinion in biotechnology 20011201
-
Purification, molecular cloning, and properties of a beta-glycosidase isolated from midgut lumen of Tenebrio molitor (Coleoptera) larvae.
Insect biochemistry and molecular biology 20011001
-
Role of organic solvents on Pa-hydroxynitrile lyase interfacial activity and stability.
Biotechnology and bioengineering 20010705
-
Production of a cyanogenic secretion by a thyridid caterpillar (Calindoea trifascialis, Thyrididae, Lepidoptera).
Die Naturwissenschaften 20010701
-
Asymmetric synthesis of an (R)-cyanohydrin using enzymes entrapped in lens-shaped gels.
Organic letters 20010628
