533-48-2 | D-Desthiobiotin

Packsize	Purity	Availability	Price	Discounted Price
100mg	95%	in stock	$39.00	$27.00
250mg	95%	in stock	$63.00	$44.00
1g	95%	in stock	$176.00	$123.00
5g	95%	in stock	$459.00	$321.00
10g	95%	in stock	$888.00	$621.00
25g	95%	in stock	$1,858.00	$1,300.00

Description

• Catalog Number: AG18026
• Chemical Name: D-Desthiobiotin
• CAS Number: 533-48-2
• Molecular Formula: C10H18N2O3
• Molecular Weight: 214.2615
• MDL Number: MFCD00213804
• SMILES: OC(=O)CCCCC[C@H]1NC(=O)N[C@H]1C

Computed Properties

• Complexity: 243
• Covalently-Bonded Unit Count: 1
• Defined Atom Stereocenter Count: 2
• Heavy Atom Count: 15
• Hydrogen Bond Acceptor Count: 3
• Hydrogen Bond Donor Count: 3
• Rotatable Bond Count: 6
• XLogP3: 0.8

Literature

• Experimental Anti-Inflammatory Drug Semapimod Inhibits TLR Signaling by Targeting the TLR Chaperone gp96.

  Journal of immunology (Baltimore, Md. : 1950) 20160615

• Biotin synthase: insights into radical-mediated carbon-sulfur bond formation.

  Biochimica et biophysica acta 20121101

• Biochemical and structural characterization of the Arabidopsis bifunctional enzyme dethiobiotin synthetase-diaminopelargonic acid aminotransferase: evidence for substrate channeling in biotin synthesis.

  The Plant cell 20120401

• A continuous fluorescence displacement assay for BioA: an enzyme involved in biotin biosynthesis.

  Analytical biochemistry 20110901

• Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.

  Protein science : a publication of the Protein Society 20110701

• Affinity recovery of lentivirus by diaminopelargonic acid mediated desthiobiotin labelling.

  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20100715

• Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli.

  BMC biotechnology 20100101

• Protein residues that control the reaction trajectory in S-adenosylmethionine radical enzymes: mutagenesis of asparagine 153 and aspartate 155 in Escherichia coli biotin synthase.

  Biochemistry 20090324

• Selection of smart small-molecule ligands: the proof of principle.

  Analytical chemistry 20090101

• 9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase.

  Biochemistry 20080902

• Purification of His-tagged proteins with [desthiobiotin-BSA-EDTA] conjugates exhibiting resistance to EDTA.

  Bioconjugate chemistry 20080301

• Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin.

  Journal of bioscience and bioengineering 20080301

• A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes.

  Proteomics 20071201

• In vivo resolution of conflicting in vitro results: synthesis of biotin from dethiobiotin does not require pyridoxal phosphate.

  Chemistry & biology 20071101

• The ISC [corrected] proteins Isa1 and Isa2 are required for the function but not for the de novo synthesis of the Fe/S clusters of biotin synthase in Saccharomyces cerevisiae.

  Eukaryotic cell 20070301

• Fluorescence-activated cell sorting and cloning of bona fide CD8+ CTL with reversible MHC-peptide and antibody Fab' conjugates.

  Journal of immunology (Baltimore, Md. : 1950) 20060915

• Biological significance and development of practical synthesis of biotin.

  Medicinal research reviews 20060701

• Microsphere-based protease assays and screening application for lethal factor and factor Xa.

  Cytometry. Part A : the journal of the International Society for Analytical Cytology 20060501

• Biotin synthase mechanism: an overview.

  Biochemical Society transactions 20050801

• A nucleosidase required for in vivo function of the S-adenosyl-L-methionine radical enzyme, biotin synthase.

  Chemistry & biology 20050501

• Biotin synthase is catalytic in vivo, but catalysis engenders destruction of the protein.

  Chemistry & biology 20050401

• Further investigation on the turnover of Escherichia coli biotin synthase with dethiobiotin and 9-mercaptodethiobiotin as substrates.

  Biochemistry 20041228

• Development of an enzymatic method for site-specific incorporation of desthiobiotin to recombinant proteins in vitro.

  Analytical biochemistry 20040815

• Role of the [2Fe-2S] cluster in recombinant Escherichia coli biotin synthase.

  Biochemistry 20040224

• Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme.

  Science (New York, N.Y.) 20040102

• Control of adenosylmethionine-dependent radical generation in biotin synthase: a kinetic and thermodynamic analysis of substrate binding to active and inactive forms of BioB.

  Biochemistry 20030311

• Biotin synthase of Bacillus subtilis shows less reactivity than that of Escherichia coli in in vitro reaction systems.

  Archives of microbiology 20021201

• Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.

  Analytical biochemistry 20020915

• Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli.

  The Journal of biological chemistry 20020419

• Biocytin and biotin uptake into NB2a neuroblastoma and C6 astrocytoma cells.

  Brain research 20020125